Questions on the method used for the study. The first bacterial cell did not have any resistance. This was marked in a color to identify its origin right?
You mentioned that several plates were used in the experiment. Were the bacterial cells from the first generation of clones that were marked in the first plate used for all other plates? Are the clones from the first cell easily identified to determine what generation produced the resistance on each plate. or are the other plates used for each generation produced from the first plate?
If only a few of the colonies survived, do you mean the entire plate or is it individuals in the colonies on each plate that survived?
Can the study pinpoint exactly when it occurred for resistance in generation time or the original bacteria cell?
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