Understanding through Discussion


Welcome! You are not logged in. [ Login ]
EvC Forum active members: 111 (8738 total)
Current session began: 
Page Loaded: 04-25-2017 2:36 AM
387 online now:
CRR, Heathen, PaulK, ThinAirDesigns (4 members, 383 visitors)
Chatting now:  Chat room empty
Newest Member: Jayhawker Soule
Post Volume:
Total: 805,155 Year: 9,761/21,208 Month: 2,848/2,674 Week: 272/961 Day: 2/134 Hour: 0/1

Announcements: Reporting debate problems OR discussing moderation actions/inactions


Thread  Details

Email This Thread
Newer Topic | Older Topic
  
Prev1
2
3Next
Author Topic:   Decisions, decisions......
Tanypteryx
Member
Posts: 1344
From: Oregon, USA
Joined: 08-27-2006
Member Rating: 6.3


Message 16 of 44 (790988)
09-09-2016 12:28 AM
Reply to: Message 15 by Coyote
09-08-2016 11:42 PM


Re: Decisions Decisions
Good for you!
We're doing science now!

Thanks!

Ain't life cool !!

The anti-science crowd will never get it.


What if Eleanor Roosevelt had wings? -- Monty Python

One important characteristic of a theory is that is has survived repeated attempts to falsify it. Contrary to your understanding, all available evidence confirms it. --Subbie

If evolution is shown to be false, it will be at the hands of things that are true, not made up. --percy


This message is a reply to:
 Message 15 by Coyote, posted 09-08-2016 11:42 PM Coyote has not yet responded

Replies to this message:
 Message 17 by Faith, posted 09-09-2016 12:35 AM Tanypteryx has responded

    
Faith
Member
Posts: 24398
Joined: 10-06-2001
Member Rating: 1.0


(1)
Message 17 of 44 (790990)
09-09-2016 12:35 AM
Reply to: Message 16 by Tanypteryx
09-09-2016 12:28 AM


Re: Decisions Decisions
I just want to say I'm enjoying this thread -- you've made the kind of science you do sound exciting and important. I also enjoy your reports and photos of dragonflies. I'm happy to know about these things.

Edited by Faith, : No reason given.


This message is a reply to:
 Message 16 by Tanypteryx, posted 09-09-2016 12:28 AM Tanypteryx has responded

Replies to this message:
 Message 18 by Tanypteryx, posted 09-09-2016 1:03 AM Faith has not yet responded
 Message 24 by caffeine, posted 09-10-2016 2:57 PM Faith has not yet responded

    
Tanypteryx
Member
Posts: 1344
From: Oregon, USA
Joined: 08-27-2006
Member Rating: 6.3


Message 18 of 44 (790992)
09-09-2016 1:03 AM
Reply to: Message 17 by Faith
09-09-2016 12:35 AM


Re: Decisions Decisions
That's nice of you to say. I'm glad you enjoy it.

What if Eleanor Roosevelt had wings? -- Monty Python

One important characteristic of a theory is that is has survived repeated attempts to falsify it. Contrary to your understanding, all available evidence confirms it. --Subbie

If evolution is shown to be false, it will be at the hands of things that are true, not made up. --percy


This message is a reply to:
 Message 17 by Faith, posted 09-09-2016 12:35 AM Faith has not yet responded

    
New Cat's Eye
Member
Posts: 11236
From: near St. Louis
Joined: 01-27-2005
Member Rating: 1.8


(1)
Message 19 of 44 (791033)
09-09-2016 10:49 AM
Reply to: Message 14 by Tanypteryx
09-08-2016 11:38 PM


Re: Decisions Decisions
I am going to accept the job

Awesome! Congratulations.

Yep, it is 720 hours, so basically I can work 720 hours between now and the end of the year and the another 720 in 2017,

Okay, that ain't so bad. You could work three days a week for five hours each day and work all year.

When I got home my wife asked me if I would really like to go back to work full time and the truth is, I would.

When my dad retired he spent two weeks on the couch watching TV and then said fuck this and went out and found a job.

It was an incredibly rewarding job and every day brought new challenges and every day I learned new things.

...

When I started there the position of Insect Imaging Specialist did not exist. Now there are 2 full time people and 2 support staff and there are almost more imaging projects than they can handle. Lots of the images are used in computer based insect screening aids that are distributed to scientists and technicians at federal and state agencies to help them identify native species, but more importantly exotic invasive species.

Yeah, that sounds really cool. Good for you!


This message is a reply to:
 Message 14 by Tanypteryx, posted 09-08-2016 11:38 PM Tanypteryx has responded

Replies to this message:
 Message 20 by Tanypteryx, posted 09-09-2016 11:16 AM New Cat's Eye has responded

  
Tanypteryx
Member
Posts: 1344
From: Oregon, USA
Joined: 08-27-2006
Member Rating: 6.3


(1)
Message 20 of 44 (791035)
09-09-2016 11:16 AM
Reply to: Message 19 by New Cat's Eye
09-09-2016 10:49 AM


Re: Decisions Decisions
Awesome! Congratulations.

Thanks!

When my dad retired he spent two weeks on the couch watching TV and then said fuck this and went out and found a job.

Yeah, the couch and TV are a deadly combination.

I just wish I was 30 years younger (well, don't we all?). The photo-techniques I use are only possible using digital cameras. I wish they and the whole computer revolution had come along 30 years earlier.

The technology existed 30 years earlier but not in the form of products anyone can own.

I remember watch TV when the images were being received from the Voyager spacecraft as they flew by Saturn. The images didn't look like much at first, but then they were "enhanced" using the huge, multi-million dollar computer at JPL and suddenly we had those absolutely amazing shots of the rings. My friends and I looked at each other and said "someday we'll be able to do that."

Here we are 40 years later and we can do SO much more.


What if Eleanor Roosevelt had wings? -- Monty Python

One important characteristic of a theory is that is has survived repeated attempts to falsify it. Contrary to your understanding, all available evidence confirms it. --Subbie

If evolution is shown to be false, it will be at the hands of things that are true, not made up. --percy


This message is a reply to:
 Message 19 by New Cat's Eye, posted 09-09-2016 10:49 AM New Cat's Eye has responded

Replies to this message:
 Message 21 by New Cat's Eye, posted 09-09-2016 11:25 AM Tanypteryx has acknowledged this reply

    
New Cat's Eye
Member
Posts: 11236
From: near St. Louis
Joined: 01-27-2005
Member Rating: 1.8


(4)
Message 21 of 44 (791037)
09-09-2016 11:25 AM
Reply to: Message 20 by Tanypteryx
09-09-2016 11:16 AM


Re: Decisions Decisions
I just wish I was 30 years younger (well, don't we all?)

No because I'd be 5


This message is a reply to:
 Message 20 by Tanypteryx, posted 09-09-2016 11:16 AM Tanypteryx has acknowledged this reply

  
NoNukes
Member
Posts: 9431
From: Central NC USA
Joined: 08-13-2010
Member Rating: 2.8


(1)
Message 22 of 44 (791040)
09-09-2016 12:35 PM
Reply to: Message 14 by Tanypteryx
09-08-2016 11:38 PM


Re: Decisions Decisions
Anyway, Thanks! You guys are great!

My interests are completely selfish. I am thinking that now you go back to work for a bit, learn how to use some more equipment, maybe buy some for yourself, and I get to look at even more spectacular pictures of dragonfly cop pairs!

Glad things worked out for you!


Under a government which imprisons any unjustly, the true place for a just man is also in prison. Thoreau: Civil Disobedience (1846)

History will have to record that the greatest tragedy of this period of social transition was not the strident clamor of the bad people, but the appalling silence of the good people. Martin Luther King

I never considered a difference of opinion in politics, in religion, in philosophy, as cause for withdrawing from a friend. Thomas Jefferson


This message is a reply to:
 Message 14 by Tanypteryx, posted 09-08-2016 11:38 PM Tanypteryx has responded

Replies to this message:
 Message 23 by Tanypteryx, posted 09-09-2016 4:10 PM NoNukes has acknowledged this reply

    
Tanypteryx
Member
Posts: 1344
From: Oregon, USA
Joined: 08-27-2006
Member Rating: 6.3


(1)
Message 23 of 44 (791057)
09-09-2016 4:10 PM
Reply to: Message 22 by NoNukes
09-09-2016 12:35 PM


Re: Decisions Decisions
I am thinking that now you go back to work for a bit, learn how to use some more equipment, maybe buy some for yourself, and I get to look at even more spectacular pictures of dragonfly cop pairs!

And not just dragonflies. I will post some of the totally bizarre insects I am shooting. For example, some of the bark beetles (Scolytines) are not much bigger than a period on a printed page, but they have amazing sculpturing on their exoskeleton, function unknown. We can have all sorts of interesting discussions about how these various features evolved.


What if Eleanor Roosevelt had wings? -- Monty Python

One important characteristic of a theory is that is has survived repeated attempts to falsify it. Contrary to your understanding, all available evidence confirms it. --Subbie

If evolution is shown to be false, it will be at the hands of things that are true, not made up. --percy


This message is a reply to:
 Message 22 by NoNukes, posted 09-09-2016 12:35 PM NoNukes has acknowledged this reply

    
caffeine
Member
Posts: 1252
From: Prague, Czech Republic
Joined: 10-22-2008
Member Rating: 3.9


(3)
Message 24 of 44 (791104)
09-10-2016 2:57 PM
Reply to: Message 17 by Faith
09-09-2016 12:35 AM


Re: Decisions Decisions
I just want to say I'm enjoying this thread(..)

Personally, I'm reading about someone choosing between continuing an enjoyable retirement or returning to a job they love, and am overwhelmed with envy. I guess you're a more magnanumous person than I.


This message is a reply to:
 Message 17 by Faith, posted 09-09-2016 12:35 AM Faith has not yet responded

  
Tanypteryx
Member
Posts: 1344
From: Oregon, USA
Joined: 08-27-2006
Member Rating: 6.3


(3)
Message 25 of 44 (792063)
10-02-2016 2:43 PM


The honeymoon is over
I spent the last 3 weeks working with the fellow who has had the position for the last 4 years. It was really fun learning his techniques and methods of shooting and processing images.

We each have completely different workflows and I think learned a lot from each other.

Friday was his last day, so tomorrow I am on my own, continuing work on a large shooting project he started, including processing several hundred images he had already shot.

It was odd to see as he was packing up his personal stuff that he had left a lot of things exactly as I had left them, as if he was afraid to make the work space his own. It was nice that he had put up a lot of his artwork and photos on the walls though.

I can only work until next March 15 if I work 40 hour weeks. I am hoping that even if they hire someone during that period I can work that long to train them on the equipment. That should give me enough extra cash to buy a new camera body and several new microscope objective lenses.


What if Eleanor Roosevelt had wings? -- Monty Python

One important characteristic of a theory is that is has survived repeated attempts to falsify it. Contrary to your understanding, all available evidence confirms it. --Subbie

If evolution is shown to be false, it will be at the hands of things that are true, not made up. --percy


Replies to this message:
 Message 26 by NoNukes, posted 10-02-2016 3:59 PM Tanypteryx has responded
 Message 27 by Taq, posted 10-03-2016 3:42 PM Tanypteryx has responded

    
NoNukes
Member
Posts: 9431
From: Central NC USA
Joined: 08-13-2010
Member Rating: 2.8


Message 26 of 44 (792066)
10-02-2016 3:59 PM
Reply to: Message 25 by Tanypteryx
10-02-2016 2:43 PM


Re: The honeymoon is over
That should give me enough extra cash to buy a new camera body and several new microscope objective lenses

Hear, hear!! I sincerely hope that's what happens!

I suppose this isn't the time to ask about those woolly mammoths?


Under a government which imprisons any unjustly, the true place for a just man is also in prison. Thoreau: Civil Disobedience (1846)

History will have to record that the greatest tragedy of this period of social transition was not the strident clamor of the bad people, but the appalling silence of the good people. Martin Luther King

I never considered a difference of opinion in politics, in religion, in philosophy, as cause for withdrawing from a friend. Thomas Jefferson

Seems to me if its clear that certain things that require ancient dates couldn't possibly be true, we are on our way to throwing out all those ancient dates on the basis of the actual evidence. -- Faith


This message is a reply to:
 Message 25 by Tanypteryx, posted 10-02-2016 2:43 PM Tanypteryx has responded

Replies to this message:
 Message 29 by Tanypteryx, posted 10-04-2016 9:52 PM NoNukes has not yet responded

    
Taq
Member
Posts: 6051
Joined: 03-06-2009
Member Rating: 3.6


(3)
Message 27 of 44 (792107)
10-03-2016 3:42 PM
Reply to: Message 25 by Tanypteryx
10-02-2016 2:43 PM


Re: The honeymoon is over
Tanypteryx writes:

That should give me enough extra cash to buy a new camera body and several new microscope objective lenses.

I can understand the enthusiasm. I have spent time using a laser confocal microscope in a research setting (600x magnification). It was extremely frustrating trying to get my fluorescent antibodies to work, but the process of taking pictures was really fun. I spent quite a few hours one time taking z-slices of our test slide (plant tissue stained with fluorescent dyes) to make these really cool 3D reconstructions that I could animate. Of course, it was all in the name of familiarizing myself with the hardware and software . There were many times when I wished I could just be taking pretty pictures instead of trying to get data.

It sounds like you are using much lower power objectives than I am, but the objectives do make all the difference in the world when it comes to higher magnifications. I am assuming the same is true for your equipment. Sometimes I will look through an older or less expensive microscope and realize how spoiled I am at work.

Edited by Taq, : No reason given.


This message is a reply to:
 Message 25 by Tanypteryx, posted 10-02-2016 2:43 PM Tanypteryx has responded

Replies to this message:
 Message 28 by Tanypteryx, posted 10-04-2016 9:49 PM Taq has responded

  
Tanypteryx
Member
Posts: 1344
From: Oregon, USA
Joined: 08-27-2006
Member Rating: 6.3


(1)
Message 28 of 44 (792128)
10-04-2016 9:49 PM
Reply to: Message 27 by Taq
10-03-2016 3:42 PM


Re: The honeymoon is over
It sounds like you are using much lower power objectives than I am, but the objectives do make all the difference in the world when it comes to higher magnifications. I am assuming the same is true for your equipment.

Yes, my objectives are lower power than that. Mitutoyo Infinity Corrected Long Working Distance Objectives

I want a 5X, 10X, and 20X. It is surprising, though how much you can enlarge a very small subject when you have high resolution optics mounted on a good camera.

We have a very nice Nikon compound scope that I want to use to photograph small pond life, but people are often surprised to find out that some of the shots we get were not from that scope but instead we from our Mitutoyo 20x mounted in front of a 150mm macro lens.

My plan for my personal imaging system will be to mount the Mitutoyo objectives in front of my 200mm micro-Nikkor on a Nikon D810. The whole thing will be mounted on a Stackshot which I already have. STACKSHOT 3X EXTENDED MACRO RAIL

This will all be mounted on a 300 lb steel optical bench for horizontal shooting or on heavy duty steel vertical carriage system for shooting vertically.

Sometimes I will look through an older or less expensive microscope and realize how spoiled I am at work.

Me too, but if I really need more magnification I will still be able to use the SEM at work. I am building a bunch of good will by helping them out right now. We have gotten some stunning images at 25,000X with it.

Edited by Tanypteryx, : No reason given.


What if Eleanor Roosevelt had wings? -- Monty Python

One important characteristic of a theory is that is has survived repeated attempts to falsify it. Contrary to your understanding, all available evidence confirms it. --Subbie

If evolution is shown to be false, it will be at the hands of things that are true, not made up. --percy


This message is a reply to:
 Message 27 by Taq, posted 10-03-2016 3:42 PM Taq has responded

Replies to this message:
 Message 30 by Taq, posted 10-05-2016 6:23 PM Tanypteryx has not yet responded

    
Tanypteryx
Member
Posts: 1344
From: Oregon, USA
Joined: 08-27-2006
Member Rating: 6.3


Message 29 of 44 (792129)
10-04-2016 9:52 PM
Reply to: Message 26 by NoNukes
10-02-2016 3:59 PM


Re: The honeymoon is over
I suppose this isn't the time to ask about those woolly mammoths?

I will try and get some mammoth photos post this coming weekend. I got kind of sidetracked.


What if Eleanor Roosevelt had wings? -- Monty Python

One important characteristic of a theory is that is has survived repeated attempts to falsify it. Contrary to your understanding, all available evidence confirms it. --Subbie

If evolution is shown to be false, it will be at the hands of things that are true, not made up. --percy


This message is a reply to:
 Message 26 by NoNukes, posted 10-02-2016 3:59 PM NoNukes has not yet responded

    
Taq
Member
Posts: 6051
Joined: 03-06-2009
Member Rating: 3.6


Message 30 of 44 (792152)
10-05-2016 6:23 PM
Reply to: Message 28 by Tanypteryx
10-04-2016 9:49 PM


Re: The honeymoon is over
Tanypteryx writes:

Yes, my objectives are lower power than that. Mitutoyo Infinity Corrected Long Working Distance Objectives
I want a 5X, 10X, and 20X. It is surprising, though how much you can enlarge a very small subject when you have high resolution optics mounted on a good camera.

We have a very nice Nikon compound scope that I want to use to photograph small pond life, but people are often surprised to find out that some of the shots we get were not from that scope but instead we from our Mitutoyo 20x mounted in front of a 150mm macro lens.

My plan for my personal imaging system will be to mount the Mitutoyo objectives in front of my 200mm micro-Nikkor on a Nikon D810. The whole thing will be mounted on a Stackshot which I already have. STACKSHOT 3X EXTENDED MACRO RAIL
This will all be mounted on a 300 lb steel optical bench for horizontal shooting or on heavy duty steel vertical carriage system for shooting vertically.

You had me at field planarity.

We are using a Leica system with objectives that have color correction specific to fluorescent spectroscopy and top of the line field planarity and a floating optical bench that weighs a ton. Both the stage and objectives are motorized which makes a world of difference. If you are curious, here is the brochure for the range of Leica objectives used in biological research:

https://www.leica-microsystems.com/...ective-Brochure_EN.pdf

Your expertise definitely outdistances mine when it comes to optics, but it is something I can geek out on. I wish we had access to SEM or TEM, but maybe some day in the future. One can only hope.


This message is a reply to:
 Message 28 by Tanypteryx, posted 10-04-2016 9:49 PM Tanypteryx has not yet responded

  
Prev1
2
3Next
Newer Topic | Older Topic
Jump to:


Copyright 2001-2015 by EvC Forum, All Rights Reserved

™ Version 4.0 Beta
Innovative software from Qwixotic © 2017