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Author Topic:   "junk DNA" a useful term or not?
gnojek
Inactive Member


Message 31 of 33 (211156)
05-25-2005 1:46 PM
Reply to: Message 29 by Dead Parrot
05-23-2005 3:45 AM


Re: DIY DNA extraction!
Well, that would extract a lot of things like membranes and proteins and such wouldn't it?
Your DNA would likely be in there, but this doesn't sound like any way to purify it or anything.
And to the above poster, there is tons of science programming.
What about the Discovery channel, the Science channel, Animal Planet, and shows on PBS?

This message is a reply to:
 Message 29 by Dead Parrot, posted 05-23-2005 3:45 AM Dead Parrot has not replied

Replies to this message:
 Message 33 by Hrun, posted 06-01-2005 1:10 AM gnojek has not replied

  
judge
Member (Idle past 6443 days)
Posts: 216
From: australia
Joined: 11-11-2002


Message 32 of 33 (212428)
05-29-2005 9:45 PM


I'm sure this company is hoping "junk DNA" is not junk. If I understsnd they patented some or a lot of junk DNA.
http://www.gtg.com.au/
Some people think pseudo genes should really be called potogenes.
quote:
Pseudogenes have been defined as nonfunctional sequences of genomic DNA originally derived from functional genes. It is therefore assumed that all pseudogene mutations are selectively neutral and have equal probability to become fixed in the population. Rather, pseudogenes that have been suitably investigated often exhibit functional roles, such as gene expression, gene regulation, generation of genetic (antibody, antigenic, and other) diversity. Pseudogenes are involved in gene conversion or recombination with functional genes. Pseudogenes exhibit evolutionary conservation of gene sequence, reduced nucleotide variability, excess synonymous over nonsynonymous nucleotide polymorphism, and other features that are expected in genes or DNA sequences that have functional roles. We first review the Drosophila literature and then extend the discussion to the various functional features identified in the pseudogenes of other organisms. A pseudogene that has arisen by duplication or retroposition may, at first, not be subject to natural selection if the source gene remains functional. Mutant alleles that incorporate new functions may, nevertheless, be favored by natural selection and will have enhanced probability of becoming fixed in the population. We agree with the proposal that pseudogenes be considered as potogenes, i.e., DNA sequences with a potentiality for becoming new genes.
Just a moment...
This message has been edited by judge, 05-29-2005 09:48 PM
This message has been edited by judge, 05-29-2005 09:50 PM
This message has been edited by judge, 05-29-2005 09:53 PM

  
Hrun
Inactive Member


Message 33 of 33 (212964)
06-01-2005 1:10 AM
Reply to: Message 31 by gnojek
05-25-2005 1:46 PM


Re: DIY DNA extraction!
gnojek writes:
Well, that would extract a lot of things like membranes and proteins and such wouldn't it?
Your DNA would likely be in there, but this doesn't sound like any way to purify it or anything.
Indeed, membranes and proteins would also be extracted. However, the procedure described by Dead Parrot does not claim to purify the DNA, merely to visualize it. And it does so because at the interphase between the salt water and the alcohol DNA becomes insouble and precipitates as stringy white clumps.

This message is a reply to:
 Message 31 by gnojek, posted 05-25-2005 1:46 PM gnojek has not replied

  
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