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Author Topic:   Is ID Scientific? (was "Abusive Assumptions")
Trixie
Member (Idle past 3728 days)
Posts: 1011
From: Edinburgh
Joined: 01-03-2004


Message 134 of 292 (229810)
08-04-2005 4:37 PM
Reply to: Message 131 by Evopeach
08-04-2005 3:07 PM


Amino acids in DNA
I've just stumbled upon this thread and I'm intrigued as to where you're sticking your amino acids in your DNA. As far as I'm aware DNA isn't a long string of amino acids. I thought it was a long string of nucleotide bases, not amino acids at all and a really funny thought struck me. When I use the DNA synthesiser to make oligonucleotides and I don't provide any amino acids, I still get DNA oligonucleotides out the other end. When I do PCR and add nucleotide building blocks to my reactions, but don't add amino acids, I still get DNA produced at the end.
Heck, I musta bin doing it wrong for...........oh years and years!
Or maybe you really don't have a clue what you're talking about.
My experience tells me it's the latter.

This message is a reply to:
 Message 131 by Evopeach, posted 08-04-2005 3:07 PM Evopeach has not replied

Trixie
Member (Idle past 3728 days)
Posts: 1011
From: Edinburgh
Joined: 01-03-2004


Message 167 of 292 (230289)
08-05-2005 4:57 PM
Reply to: Message 163 by Evopeach
08-05-2005 3:30 PM


Re: Fundamental lack of understanding of molecular biology
Er........you did, actually and I read it exactly the same way as WK and Asgara. I suggest you go back and re-read your posts and you'll see where you made this erroneous claim.
You also seem to be mixing up the replication of DNA and protein synthesis. DNA replication doesn't involve transcription of DNA to messenger RNA - it's protein synthesis that requires that.
I really would advise you to do a bit of reading on this before making such statements. That way your arguments might be a bit more informed and a bit less dependent on your erroneous comprehension of the subject.

This message is a reply to:
 Message 163 by Evopeach, posted 08-05-2005 3:30 PM Evopeach has replied

Replies to this message:
 Message 168 by Evopeach, posted 08-05-2005 6:14 PM Trixie has replied

Trixie
Member (Idle past 3728 days)
Posts: 1011
From: Edinburgh
Joined: 01-03-2004


Message 183 of 292 (230511)
08-06-2005 4:32 PM
Reply to: Message 168 by Evopeach
08-05-2005 6:14 PM


Re: Fundamental lack of understanding of molecular biology
While protein synthesis might be going on during DNA replication, it is not an absolute requirement of DNA synthesis. If the necessary proteins are present in sufficient quantities then DNA replication will occur without protein synthesis.
in vitro, DNA synthesis occurs with absolutely no protein synthesis. Its called PCR and is a standard technique in labs the world over.
Yes, in vivo certain enzymes are required to uncoil, unzip and rezip the DNA, but their synthesis doesn't have to be during replication. So, although DNA synthesis and protein replication are linked and both can occur at the same time, they are considered two different processes.
In science, you have to look at systems stripped to the bare essentials if you want to understand the mechanisms involved. However, scientists are well aware that when they look at a particular stripped-down system, they're looking at a stripped-down system (mainly because they stripped it down in the first place before they started). We know that all the systems are interlinked in all sorts of ways. However we also have a basic understanding of what is going on in each of these systems and that's why we don't tend to add amino acids to our PCR reactions.
If you do a search on the internet you can find molecular diagrams of amino acids and nucleotide bases and you'll see that amino acids could never take the place of purines and pyrimidines.

This message is a reply to:
 Message 168 by Evopeach, posted 08-05-2005 6:14 PM Evopeach has not replied

Replies to this message:
 Message 185 by Wounded King, posted 08-06-2005 6:57 PM Trixie has replied

Trixie
Member (Idle past 3728 days)
Posts: 1011
From: Edinburgh
Joined: 01-03-2004


Message 184 of 292 (230520)
08-06-2005 4:53 PM
Reply to: Message 179 by Admin
08-06-2005 12:00 PM


Re: Fundamental lack of understanding of molecular biology
Hi Percy, I understand that you may be a bit concerned about the on topicness or otherwise of this, but I feel that some of the comments on DNA replication and, especially amino acids in DNA, are most definitely on topic.
The topic title is "Is ID Scientific?". Quite frankly, if proponents are trying to defend their position by wittering on about amino acids being the four bases in DNA, then the answer to the question is a resounding "NO!!!"
To try to take on the intricacies of the cellular systems while having serious flaws in the understanding of even small parts of the systems is like pushing jelly uphill.

This message is a reply to:
 Message 179 by Admin, posted 08-06-2005 12:00 PM Admin has not replied

Trixie
Member (Idle past 3728 days)
Posts: 1011
From: Edinburgh
Joined: 01-03-2004


Message 186 of 292 (230736)
08-07-2005 4:53 PM
Reply to: Message 185 by Wounded King
08-06-2005 6:57 PM


Re: Fundamental lack of understanding of molecular biology
Hi WK, you quoted Evopeach as saying
I said there were no r form amino acids involved in the dna molecules genetic code and for good reason they wouldn't fit, they couldn't code for the correct proteins even if they could be fit into DNA they couldn't be read from an "mrna" strand by the ribosome enzyme and thus the replication would fail, period.
(bold text mine)
This states that Evopeach believes that without protein synthesis, replication won't happen. I merely pointed out that while proteins might be needed, their synthesis doesn't have to happen during DNA replication.
As to PCR reactions I never claimed that enzymes weren't required in PCR, I claimed that the synthesis of those enzymes wasn't required. As you said yourself, you add Taq, you don't have it being synthesised in the reaction. It may seem like a minor point, but I never claimed that enzymes weren't required, just their synthesis.
Every time Evopeach has mentioned the mechanism of DNA replication, mRNA and ribosomes are brought in. You yourself suggested he had a problem with confounding the two.
Admin quotes Evopeach as saying
As to whether there is transcription of the bases after the division of DNA into two strands, sense and antisense, by a protein (RNA polymerase) which uncoils the helix. The RNA polymerase is also acting on the sense strand and spining out a strand of mrna, note mrna. It is transferring information from the DNA to the ribosome so that a particular protein called helicase can be made it being absolutely vital to the replication of DNA. Helicase of course is that enzyme which separates the DNA strands for replication and along with DNA polymerase asists in the formation of a new DNA strand from free floating bases. Topoisomerase another enzyme/protein is holding tension on the yet to be unwound DNA a mechanical chore if you will.
then asks
If I understand you, you're saying that helicase is produced as a byproduct of the replication process. The question this raises is that since helicase is essential to splitting the DNA helix, at least some helicase must have been present before the splitting process began. Is your point that some of the helicase produced during replication is fed back to the process to help it continue?
which suggests to me that Admin also interpret Evopeach to be saying that the processes are concurrent.
To be perfectly honest, Evopeach has used jargon that he doesn't understand to try to make his points and then backpedals furiously when his mistakes are pointed out. For example another minor error is that he says
and the ribosome reading the codons three at a time
It's just a minor quibble, but the ribosome reads the bases three at a time and the three bases are called a codon. Reading codons three at a time means reading nine bases at a time.
I agree with you that he's just trying the old irreducibility argument, but he's doing it in such a way that he demonstrates his own shallow knowledge of the subject he expounds on.

This message is a reply to:
 Message 185 by Wounded King, posted 08-06-2005 6:57 PM Wounded King has not replied

Replies to this message:
 Message 188 by Evopeach, posted 08-07-2005 8:32 PM Trixie has not replied

Trixie
Member (Idle past 3728 days)
Posts: 1011
From: Edinburgh
Joined: 01-03-2004


Message 258 of 292 (231522)
08-09-2005 4:37 PM
Reply to: Message 254 by Evopeach
08-09-2005 3:51 PM


Re: Talking Points
You said
Is carbon 12 irreducibly complex of course because if you remove the electrons, protons or neutrons you no longer have a carbon atom. I did not say one electron or whatever, as between isotopes, I said all of any one type of particle whichever.
You also said
Now we have identified a component ubiquitous throughout each and every living entity, carbon ,which is itself irreducibly complex.
It is apparent that the designer used a unique and particular component for creating every form of life and that the component is not reducable itself if it is to continue its function and idenity.
Are you trying to say that carbon alone of all the elements is IC and the others aren't? Surely the same applies to them. Does this mean that nitrogen has been intelligently designed? Oxygen, calcium, silicon? What about compounds like NaCl? Intelligently designed salt???????????
Edited to put in missing quote
This message has been edited by Trixie, 08-09-2005 04:47 PM

This message is a reply to:
 Message 254 by Evopeach, posted 08-09-2005 3:51 PM Evopeach has not replied

Trixie
Member (Idle past 3728 days)
Posts: 1011
From: Edinburgh
Joined: 01-03-2004


Message 262 of 292 (231539)
08-09-2005 5:13 PM
Reply to: Message 259 by Evopeach
08-09-2005 4:57 PM


Re: Talking Points
As you said and I quoted above
It is apparent that the designer used a unique and particular component for creating every form of life and that the component is not reducable itself if it is to continue its function and idenity.
Have you thought of removing all the hydrogen? How about nitrogen? How about phosporous? How about oxygen? If you get a different outcome then your hypothesis of carbon being unique and particular survives. If you get the same outcome your hypothesis falls flat on it's face.
I predict that the outcome will be the same.

This message is a reply to:
 Message 259 by Evopeach, posted 08-09-2005 4:57 PM Evopeach has replied

Replies to this message:
 Message 269 by Evopeach, posted 08-09-2005 6:05 PM Trixie has not replied

Trixie
Member (Idle past 3728 days)
Posts: 1011
From: Edinburgh
Joined: 01-03-2004


Message 263 of 292 (231542)
08-09-2005 5:21 PM
Reply to: Message 261 by Evopeach
08-09-2005 5:12 PM


Re: Talking Points
So uranium isn't an element. Glad we cleared that up. There are only five elements in the periodic table. Erm..........what are the rest of the "things" in the periodic table?
How about potassium, sodium, calcium?
How about iron? Sulphur? If you remove these a heck of a lot of life forms will pop their clogs.

This message is a reply to:
 Message 261 by Evopeach, posted 08-09-2005 5:12 PM Evopeach has not replied

Trixie
Member (Idle past 3728 days)
Posts: 1011
From: Edinburgh
Joined: 01-03-2004


Message 290 of 292 (231631)
08-09-2005 7:39 PM
Reply to: Message 286 by Evopeach
08-09-2005 7:03 PM


Re: Talking Points
Evopeach, how much biology and chemistry have you studied?

This message is a reply to:
 Message 286 by Evopeach, posted 08-09-2005 7:03 PM Evopeach has not replied

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