That seems reasonable, however we need to be sure that the mutation is actually in a gene that codes a protein.
I could not find any info on the specific mutation that allowed these bacteria to digest citrate. How do you know it was not a mutation to a gene controller?
Well, if you look at the DNA directly you can see where the mutation is and so what it's affecting, whether it's in protein-coding DNA or something else.
I'm not sure why you think it matters, can you expound on this?
How do you know that the enzyme that nylon eating bacterium used to break down nylon wasn't formerly used for something else?
Well, there aren't bacteria using it for something else.
That seems reasonable but it doesn't rule out gene switching.
Well, yes it does. Why do you think it doesn't?
If gene X is switched on as a consequence of environmental stimulus Y, then you'd see the whole bacterial culture switching on gene X in the first generation exposed to stimulus Y.
You mention how a crude mutation is modified to produce multicellular. This example seems like the silver bullet I am looking for. What type of genes were these mutations happening in?
Genes controlling cell division, natch. I can't find the full text of the relevant articles, just abstracts, but I don't think the researchers did look at the DNA, that's why I gave it as an example of something that one could deduce wasn't gene switching by other means.
The original experiment was done, so to speak, by accident. Dinoflagellates (single-celled predators) got into a culture of chlorella (single-celled prey), which was not what the experimenters wanted to happen. It was lucky for them they didn't just throw it out, but investigated what was going on.
One way chlorella can avoid predation is simply to get too big for the dinoflagellates to eat, and this is what happened.
Now, my reasons for thinking that this wasn't gene switching are as follows:
* The change was not immediate and universal.
* The first change was that cell division was interfered with to such an extent that big blobs of as many of a hundred cells were formed. This has the advantage that the chlorella couldn't be eaten; it has the disadvantage (one presumes) that the cells on the inside would find it difficult to acquire nutrients. The chlorella then tended towards an eight-celled form which was still too big to eat but in which every cell was on the outside of the cluster. Wouldn't gene switching jump to this form immediately?
* This never happens in the wild. Why not? Because in the wild chlorella is not just preyed upon by single-celled predators such as dinoflagellates. A clump of chlorella is too big for a dinofalgellate, it's a tasty meal for a fish. Why would chlorella have a genetic switch
just to cope with conditions only met with in the laboratory?
* When the eight-celled forms were transferred to an environment without dinoflagellates, their descendants continued to have the eigth-celled form. Now, this would be something unique in gene switching --- a gene which can be turned on, but can't then be turned off, and is inherited stuck in the on position.
So I think this is a good example of what I'm talking about --- in this case, even if we don't directly look at the DNA, it seems safe to conclude that we're looking at the results of mutation.