Taq writes:
We incubate it overnight and come back the next day to find a handful of colonies growing on the antibiotic plate...
I'm not sure how my thoughts should be structured for your experiment, so I'm just going to explain my question/comment.
Regardless of how these colonies developed their antibiotic resistance... they do have it now in some way.
I understand that some may not have had the resistance, and died, and therefore there are pockets of colonies on the antibiotic plate.
But, why didn't the pockets of colonies with antibiotic resistance continue to spread and take over the whole dish? What is preventing them from spreading over the rest of the antibiotic areas if they are, indeed, resistant to the antibiotic?
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