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Author Topic:   Do you really understand the mathematics of evolution?
Kleinman
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Message 1 of 6 (876287)
05-16-2020 1:37 PM


If you think you understand the mathematics of evolution, please explain the mathematics of the Kishony Mega-Plate experiment and the Lenski Long Term Evolutionary Experiment and what is the significant mathematical difference between the two experiments.

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 Message 2 by Admin, posted 05-17-2020 8:59 AM Kleinman has responded

Admin
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Message 2 of 6 (876318)
05-17-2020 8:59 AM
Reply to: Message 1 by Kleinman
05-16-2020 1:37 PM


Please describe the Kishony Mega-Plate and Lenski Long Term Evolutionary experiments and their mathematics. Provide links as references.

--Percy
EvC Forum Director

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Kleinman
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Posts: 528
From: United States
Joined: 10-06-2016
Member Rating: 1.1


(1)
Message 3 of 6 (876362)
05-18-2020 2:49 AM
Reply to: Message 2 by Admin
05-17-2020 8:59 AM


Sorry, I thought the bloggers on this forum would be familiar with these two experiments. You can learn about the Kishony Mega-Plate experiment here:
Spatiotemporal microbial evolution on antibiotic landscapes
and you can watch a video of the experiment here:
EXTRA MINUTES - SUPERBBUGS (Harvard Experiment explained)
Essentially, what these experimenters do is make a large petri dish. And on this petri dish, they put different levels of an antibiotic in bands on the dish. On the left and right edges of the petri dish, no antibiotic. As you move toward the middle of the petri dish, the antibiotic concentration is increased in each band. Bacteria are then inoculated on the petri dish which initially grows only in the drug-free region. But as colonies grow, variants appear which can grow in the next higher drug concentration region. In this experiment, the antibiotic is the selection pressure.

The Lenski Long Term evolution experiment is performed in a different manner. You can read about how this experiment performed here:
E. coli long-term evolution experiment - Wikipedia
There many other publications based on this experiment but this link gives the essential principles. Essentially, what this experiment consists of are vials which contain 10ml of glucose growth solution for bacteria. Each day 5e6 bacteria are introduced into the solution which has sufficient nutrients to support the growth of about 5e8 bacteria (about 6-7 doublings (generations) per day). By the end of the day, the glucose is used up. 1% of the bacteria are then taken from this solution and introduced into a fresh 10ml solution for the next day's growth. Every 500 generations, the bacteria are saved, their genetic sequence determined, mutations identified. In this experiment, the selection pressure is starvation (nutrient limitation). This experiment has been carried out continuously since 1988 until it was paused recently because of the Covid episode.

If you or any members of this forum know how to correctly describe the physics and mathematics of either of these experiments or know of links that do this, please provide that information.


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 Message 2 by Admin, posted 05-17-2020 8:59 AM Admin has responded

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Admin
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Posts: 12705
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Message 4 of 6 (876399)
05-18-2020 8:03 PM
Reply to: Message 3 by Kleinman
05-18-2020 2:49 AM


Kleinman writes:

If you or any members of this forum know how to correctly describe the physics and mathematics of either of these experiments or know of links that do this, please provide that information.

Were you hoping for a discussion, or is this a question you'd like answered?


--Percy
EvC Forum Director

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Kleinman
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Posts: 528
From: United States
Joined: 10-06-2016
Member Rating: 1.1


Message 5 of 6 (876411)
05-19-2020 7:52 AM
Reply to: Message 4 by Admin
05-18-2020 8:03 PM


Both.

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Admin
Director
Posts: 12705
From: EvC Forum
Joined: 06-14-2002


Message 6 of 6 (876418)
05-19-2020 9:41 AM


Thread Copied to Biological Evolution Forum
Thread copied to the Do you really understand the mathematics of evolution? thread in the Biological Evolution forum, this copy of the thread has been closed.

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