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Author Topic:   Neanderthals and Cro-Magnon
crashfrog
Member (Idle past 1489 days)
Posts: 19762
From: Silver Spring, MD
Joined: 03-20-2003


Message 34 of 87 (326084)
06-25-2006 11:05 AM
Reply to: Message 33 by Jor-el
06-24-2006 7:07 PM


Re: Bottleneck in human DNA Diversity
Logic would dictate that after so many millenia the DNA would be corrupted beyond salvage, although I may be working under a false premise when stating the above.
My guess, based on a few undergrad courses in genetics and my wife's research in insect molecular phylogenetics? It's not so much that DNA sequences become "corrupted" - nucleotide substitution isn't, to my knowledge, chemically likely in the environment of "decaying" DNA. It's that the DNA strands break into pieces.
But you can still PCR the pieces. Short strands of DNA are very stable. Assembling the whole strand from a random assortment of its pieces is a computationally intesnive, but not impossible, task. (Imagine if you had many copies of the same manuscript, each copy torn into tiny random pieces. For any two pieces that could be validly stuck together, there's probably a third piece from another copy that overlaps them, and tells you how they should be put together.)
So, not like computer data on a hard disk slowly degenerating into random 1's and 0's. More like the leaves of a book falling out of the spine and into random order.

This message is a reply to:
 Message 33 by Jor-el, posted 06-24-2006 7:07 PM Jor-el has replied

Replies to this message:
 Message 35 by NosyNed, posted 06-25-2006 12:54 PM crashfrog has not replied
 Message 36 by Jor-el, posted 06-26-2006 3:36 PM crashfrog has replied

  
crashfrog
Member (Idle past 1489 days)
Posts: 19762
From: Silver Spring, MD
Joined: 03-20-2003


Message 38 of 87 (326681)
06-26-2006 10:56 PM
Reply to: Message 36 by Jor-el
06-26-2006 3:36 PM


Re: Bottleneck in human DNA Diversity
So what you're saying is that They can't recover a complete DNA sequence but only a multitude of part's of that same strand and then by using computer modelling they try to construct a DNA model of what the original strand looked like.
That's my guess. It's the exact same technique, by the way, that is used to sequence the contemporary human genome. It turns out that you basically have to cleave genomic DNA into small pieces anyway, just to be able to sequence it sooner than a few decades.
mtDNA, from what I've read is apparently the study most used due to its' reliability. Is there a site which explains the use of these different tests and their purposes that is not so technical that I wouldn't understand a word? I've been looking but can't seem to find anything that is directed toward instructing someone in laymans terms.
The tests are usually always the same; PCR-RFLP or other kinds of tests. Basically, very simple ways to examine a single gene across multiple individuals and establish a phylogenetic tree of their relationships and shared ancestors.
I've been looking but can't seem to find anything that is directed toward instructing someone in laymans terms.
Offhand, I don't know. Maybe your best bet is to open a thread to discuss one of the really technical explanation, and ask the biologists here questions that will help it make sense to you.

This message is a reply to:
 Message 36 by Jor-el, posted 06-26-2006 3:36 PM Jor-el has not replied

  
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