Genetic variability in a bacteria species

Questions on the method used for the study. The first bacterial cell did not have any resistance. This was marked in a color to identify its origin right?
You mentioned that several plates were used in the experiment. Were the bacterial cells from the first generation of clones that were marked in the first plate used for all other plates? Are the clones from the first cell easily identified to determine what generation produced the resistance on each plate. or are the other plates used for each generation produced from the first plate?If only a few of the colonies survived, do you mean the entire plate or is it individuals in the colonies on each plate that survived?Can the study pinpoint exactly when it occurred for resistance in generation time or the original bacteria cell?

they don't skip generations is confusing to me. My understanding is bacteria replicate in generation time. When is it determined on a agar dish that the bacteria begin their second generation?If you take samples from the first dish and transfer them to another dish, is this still the first generation replicating?The study that found 2 or 3 immune ? out of billions that were grown does not specify if they all come from one generation of replication.
Isn't each row replicated considered another generation of bacteria?

I understand that during the lag phase that the cell is actively metabolizing for cell division but it also does this again in the stationary phase. What stage must be completed before samples are taken to determine antibiotic resistance?

http://www.mansfield.ohio-state.edu/~sabedon/black06.htmThis site said there are 4 stages:Lag Phase
Log Phase
Stationary Phase
Death PhaseIt also says doubling is considered a generation. It is also termed "generation" when the cell splits into 2 daughter cells.Edited by barbara, : No reason given.

That was perfect, thank you!