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Author Topic:   Application of the Scientific Method: Antibiotic Resistance
herebedragons
Member (Idle past 880 days)
Posts: 1517
From: Michigan
Joined: 11-22-2009


Message 17 of 20 (692212)
03-01-2013 9:03 AM
Reply to: Message 16 by Taq
02-28-2013 11:37 AM


Re: Basic Methodologies
Any questions?
First a comment then a question.
You did a good job of describing the methodologies of this experiment, but since you titled this thread "Application of the Scientific Method" I had hoped you would have spent a bit more time explaining how the scientific method was applied. I think it is easy for those that work with this stuff on a regular basis to take it for granted, but for those "non-science types" it is not quite as intuitive. I have seen a lot of confusion as to how to actually use the scientific method to come to conclusions.
For example, I like how you tested against competing hypotheses, but I don't think that issue would be clear to someone unfamiliar with the concept. It is critical that you test not only your hypothesis but the competing or null hypothesis. How often have you heard that a particular result is compatible with an ID hypothesis, which it may indeed be, but if the results are also compatible with the null hypothesis, then you cannot draw a conclusion based on those results. These are the types of things that maybe could be better addressed in this thread.
I'm going to put my question in a separate post.
HBD

Whoever calls me ignorant shares my own opinion. Sorrowfully and tacitly I recognize my ignorance, when I consider how much I lack of what my mind in its craving for knowledge is sighing for. But until the end of the present exile has come and terminated this our imperfection by which "we know in part," I console myself with the consideration that this belongs to our common nature. - Francesco Petrarca
"Nothing is easier than to persuade people who want to be persuaded and already believe." - another Petrarca gem.

This message is a reply to:
 Message 16 by Taq, posted 02-28-2013 11:37 AM Taq has replied

Replies to this message:
 Message 19 by Taq, posted 03-01-2013 7:24 PM herebedragons has not replied

  
herebedragons
Member (Idle past 880 days)
Posts: 1517
From: Michigan
Joined: 11-22-2009


Message 18 of 20 (692215)
03-01-2013 9:21 AM
Reply to: Message 16 by Taq
02-28-2013 11:37 AM


Re: Basic Methodologies
What are the results? We see colonies at the same position on each of the replica plates. This is consistent with random mutations, and inconsistent with non-random mutations or induced resistance
Based on this experiment we can conclude that the mutations for resistance to this antibiotic are consistent with random mutations. But is it reasonable to infer that all mutations operate under the same principals? Can the mechanisms of antibiotic resistance in prokaryotic cells be extrapolated to eukaryotic cells or metazoans? I don't think that is a reasonable leap (from this experiment alone). * which I guess is a part of the discussion about the application of the scientific method as well - recognizing the limitations of a study and proposing new directions for research. (This is probably the thing that fascinates me most about science is that finding an answer to a hypothesis doesn't resolve an issue it provides more opportunity for questions!)
So what follow up experiment would you propose to further strengthen the connection between this study and mutations in eukaryotes and/or metazoans?
HBD

Whoever calls me ignorant shares my own opinion. Sorrowfully and tacitly I recognize my ignorance, when I consider how much I lack of what my mind in its craving for knowledge is sighing for. But until the end of the present exile has come and terminated this our imperfection by which "we know in part," I console myself with the consideration that this belongs to our common nature. - Francesco Petrarca
"Nothing is easier than to persuade people who want to be persuaded and already believe." - another Petrarca gem.

This message is a reply to:
 Message 16 by Taq, posted 02-28-2013 11:37 AM Taq has replied

Replies to this message:
 Message 20 by Taq, posted 03-01-2013 7:30 PM herebedragons has not replied

  
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