Y.E.C. Model: Was there rapid evolution and speciation post flood?

It also depends on how one defines "function." Is a spacer between gene copies "functional?" Are sections of DNA that fold into secondary structures (that may or may not have an influence on expression) "functional?" etc, etc.HBD

Faith has stated that, at least some "junk DNA" is dead genes, genes that have been inactivated by mutations. I doubt she would consider that a source of new genes.HBD

For Sanger sequencing, the region of interest is amplified using PCR. A forward and reverse primer is used that bind opposite strands at the extents of the target region as in the image below. After amplification, the PCR products are cleaned and all small nucleotides and primers are removed. Then half of the purified product is put in a well with forward primer and the other half is put into a well with reverse primer. These are processed so that the resulting sequence reads are complimentary which are then aligned using software. The software automatically detects that the strands are complimentary and converts one of them. A consensus sequence is generated from the aligned sequences and this is what is reported.I'm not sure if this is convention per se, but typically the strand that is amplified by the forward primer is the strand that gets reported. When designing primer pairs, typically you try to located the forward primer at the front of the gene so that the resulting sequence will end up in the same orientation as the direction the gene is transcribed. NexGen sequencing does not use primers to initiate sequencing and relies much more on software to assemble the individual sequences (100 - 150 bp) but the convention would be the same: to report a gene sequence so that the reported sequence is in the same orientation as the gene is transcribed.Occasionally you will find a sequence reported in the opposite direction, but software will usually identify it and it can be converted to match the rest of the sequences you are working with.HBD

I have not been following this discussion super close because I just have not had time to. But I want to see if I understand your scenario and can put it into a clear narrative.God created Adam and Eve with all the necessary genes and alleles to allow their immune system to fight off any pathogen that may attempt to invade their bodies. But because of the fall, mutations began to be introduced into an otherwise perfectly functioning system. As pathogens mutated they began to overcome the human immune system (through loss of information, of course) and they were then able to cause disease. As the human immune system began to mutate, the distribution of alleles became diluted and so a smaller and smaller proportion of people had a fully functioning immune system. Occasionally, a mutation in an immune system component would allow it to defend against a newer form of a pathogen, but this is an extremely rare exception.Is this a good summary?HBD