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Author Topic:   The Recurrent Problem of Chirality
Bradcap1
Inactive Member


Message 56 of 81 (335044)
07-24-2006 11:26 PM
Reply to: Message 53 by Brad McFall
07-24-2006 9:58 PM


Re: scaling 1-D symmetries
Last time I looked it up, I read that some description of molecular forces fall off in space at 10-7 from a given atom and since there are 1-D repeats at the number 8 (ACGT*2) then the formal relation between 1-D symmetry and any community of attraction and repulsion would be found by checking a given sequence to see if it repeats a given base at intervals of 8 "downadaptivestream." A simple JAVA or BASIC program could accomplish this.
You're not getting it. Aminoacyl tRNA synthetase cannot charge tRNAs with D-amino acids. This is due to the genetic code and it's exclusive use of L-amino acids. D-aminos just can't get in due to conformational differences. L-aminos fit.
Now, the fundamental proteins that mediate replication, transcription, translation, and expression are all coded for by DNA that directs L-amino acids. These proteins have conformations that only work with L-amino acids and so on through metabolic pathways.You would have to go back to step one for these fundamental differences to change. You should be getting the idea by now.
With the term "extra-chromosomal" I was only trying to make clear that the use of this geometrization is to intedict the possible 1-D symmetries (either across a DNA strand or along one (with further connections to the host of potential coded information)).
In other words, you have no idea what you are talking about.
You babble on about things with absolutely no biological or chemical relevancy.

This message is a reply to:
 Message 53 by Brad McFall, posted 07-24-2006 9:58 PM Brad McFall has not replied

  
Bradcap1
Inactive Member


Message 57 of 81 (335046)
07-24-2006 11:32 PM
Reply to: Message 54 by Brad McFall
07-24-2006 10:10 PM


Re: scaling 1-D symmetries
the day will come when you and other will realize that I am a first class theoretical biologist. I come to my understanding admittedly in a larger circut (that has some philosophical component) than is required for phds. This is not hubris.
Ha Ha Ha! You are so full of shit! You have shown nothing but first class ignorance of the subject!
I resent your judgements somewhat and wish you really only spoke to the subject.
You've never addressed the subject. You've only thrown out your thoughts or ideas that you can't support with any observable evidence or data. In fact, your responses show nothing but a complete ignorance of this subject.

This message is a reply to:
 Message 54 by Brad McFall, posted 07-24-2006 10:10 PM Brad McFall has not replied

  
Bradcap1
Inactive Member


Message 58 of 81 (335050)
07-24-2006 11:48 PM
Reply to: Message 55 by RAZD
07-24-2006 10:36 PM


Re: scaling responses.
I'll say this slowely for you: we do not know how life started, nor how many different forms it had, all your evidence is based on what is alive today.
Show me the evidence. Was that slow enough for you? I'll say it again in case you missed it, show me the evidence.
You don't need to say this kind of thing. It adds nothing to the debate, and reflects more on you than anyone else.
I'm not saying no-one here (least of all me) is immune to this, but we should all make the attempt at being civil.
Sure, you stick to observable, reproducible observations and not speculation and I'll be civil. If you keep speculating with no evidence to back it up, I'll keep telling you you're full of shit.
The last person to brag about his background did so to his dismay. Personally I find anyone that needs to say this has lost the argument -- the argument is based on the evidence you present, not who or what you are (that is, after all, the logicall fallacy of the appeal to authority with the added ego-centrism of claiming to be the authority).
Ha Ha Please! I've presented nothing but evidence in my posts to you. Evidently you lack even basic education in biology and chemistry to undestand it. Sucks to be you.
There are many kinds of arrogance. Another kind is thinking that you know it all. If I'm looking for a teacher I'll look for one who doesn't insult my intelligence first, and claim to be an expert second.
I absolutely do not know it all. I have however dedicated the last five years of my life to this field. I don't claim to be an expert in any other field. I do know more than the layman (that includes you and Brad)about molecular biology.
If you can demonstrate why they {had} to be L-forms, I am interested.
Exactly what I've been doing.
one final word, just to make your ... posts more interesting to read, you may have noted that indenting is nor preserved in the posts, nor is there any natural paragraph formating (note to percy ...), so the only way to really identify paragraphs is to double enter line breaks.
See there, I would certainly acknowledge that you are more knowledgeable than me in posting to this group and in computers in general.
BTW, the evidence still does not fit your view.

This message is a reply to:
 Message 55 by RAZD, posted 07-24-2006 10:36 PM RAZD has replied

Replies to this message:
 Message 59 by AdminJar, posted 07-24-2006 11:56 PM Bradcap1 has replied
 Message 62 by RAZD, posted 07-25-2006 8:07 AM Bradcap1 has replied

  
Bradcap1
Inactive Member


Message 61 of 81 (335089)
07-25-2006 5:17 AM
Reply to: Message 59 by AdminJar
07-24-2006 11:56 PM


Re: Enough
Comments like "Sucks to be you" will get you a period in the TimeOut Chair.
Really? And reachng a conclusion, then going out and looking for evidence to support it does not?
This perversion of the scientific method hardly seems worthy of these "first class theoretical biologists."
Where does one get a degree in theoretical biology anyway? You see, at the University I attended, we were unjustly required to take lab courses where we had to set up experiments, accumulate data, analyze the data, then reach conclusions.
It's nice that there is someone out there to protect scam artists like the two I've been conversing with from mean old scientists like me who demand that they back up their claims with physical evidence.

This message is a reply to:
 Message 59 by AdminJar, posted 07-24-2006 11:56 PM AdminJar has not replied

Replies to this message:
 Message 65 by AdminNWR, posted 07-25-2006 9:33 AM Bradcap1 has not replied

  
Bradcap1
Inactive Member


Message 67 of 81 (336045)
07-28-2006 12:01 PM
Reply to: Message 64 by Wounded King
07-25-2006 9:13 AM


Re: you were warned. sigh.
This is not part of the process of protein synthesis at the ribosomal level however
Exactly. This is a post-translational modification. Different proteins are required to do this job. The L-amino acids incorporated into peptides required for protein synthesis have different conformations than those that modify the peptides of the cell wall.

This message is a reply to:
 Message 64 by Wounded King, posted 07-25-2006 9:13 AM Wounded King has not replied

  
Bradcap1
Inactive Member


Message 68 of 81 (336046)
07-28-2006 12:03 PM
Reply to: Message 63 by crashfrog
07-25-2006 8:14 AM


Re: scaling 1-D symmetries
And I'd rather see you address people who know how to make some sense, because you're clearly intelligent and informed, rather than get banned for trying to provoke Brad McFall into making sense, because that's not going to happen.
You got it. It does pain me though to watch someone make claims that are completely contrary to everything my professors taught me as well as to my experience in the lab.

This message is a reply to:
 Message 63 by crashfrog, posted 07-25-2006 8:14 AM crashfrog has not replied

  
Bradcap1
Inactive Member


Message 69 of 81 (336200)
07-28-2006 8:55 PM
Reply to: Message 62 by RAZD
07-25-2006 8:07 AM


(Sigh) Re: you were warned. sigh.
You have not demonstrated any evidence that is more than 50 years old. We can extrapolate that {life today} back to common ancestors of {all current life}, but that is a very small fraction of {all life that ever existed}.
You might have missed this, but the way we understand the past is by studying the present.
You and Brad evidently do not feel that the exclusive use of L-amino acids by living organisms is not evidence of common descent. I have demonstrated that the use of L-aminos is due to the proteome, which is in turn due to the transcriptome, which is ultimately due to the genome. Even if you want to point to a primordial RNA world, your opinion is not supported. If you feel that common use of amino acids is not evidence of common descent, then I am forced to conclude that you feel that homology within genomes is not evidence of common descent. Is this correct?
You have also expressed an opinion that the preference for L-aminos could be due to predator-prey relationships. The point at which l-aminos were exclusively being used would presumably have been far earlier than these relationships would have existed.
So, from a scientific standpoint, there is no evidence to support your opinion. This is what I said earlier. Until you find an organism that is/was capable of incorporating D-aminos into nascent peptides, you are merely speculating. A line of organisms capable of incorporating D-aminos would be so fundamentally different from the rest of the tree of life that it would warrant its own domain. Have you ever seen ANY fossil record suggesting that this is the case?
If you are talking about looking at this from a philosophical standpoint, yes a lineage of D-amino using organisms could have existed. But, the problem with this perspective is that there could have been an infinite amount of other possibilities such as the seeding by aliens that an earlier post jokingly referred to. The possibilities of little green men have just as much evidence to support them as does your conjecture.
Fortunately, the scientific method overcomes this obstacle by requiring observable, reproducible evidence on which opinions are based.
There is simply no evidence to support what you say.
And no, you have not demonstrated why the first forms of life {HAD} to use L-forms, all you have demonstrated is that current life {HAS} L-forms. To claim that this is evidence that it {HAD} to be is begging the question -- another logical fallacy, btw.
I was not addressing abiogenesis in my first posts. In fact, the issue that started this was that I stated that the exclusive use of L-amino acids was evidence of common descent. Common descent is not abiogenesis. You have evidently created a strawman of my statement. It appears you are the party guilty of the logical fallacy.

This message is a reply to:
 Message 62 by RAZD, posted 07-25-2006 8:07 AM RAZD has replied

Replies to this message:
 Message 71 by RAZD, posted 07-29-2006 11:37 AM Bradcap1 has replied

  
Bradcap1
Inactive Member


Message 70 of 81 (336284)
07-29-2006 9:30 AM
Reply to: Message 62 by RAZD
07-25-2006 8:07 AM


Re: you were warned. sigh.
The one amino acid not exhibiting chirality is glycine since its '"R-group" is a hydrogen atom. ... D-amino acids are often found in polypetide antibiotics.
Are you willing to bet that no bacteria will ever take advantage of the resources available when compounds like D-amino acids are used in antibiotics? Given the many surprises that bacteria have foisted on us uninformed people over the years, I wouldn't make such a bet.
An example of a non-chiral molecule (glycine) is a poor example on which to base a claim of the usage of D-amino chiral aminos.
I am surprised that you would use antibiotics to support your views for a couple of reasons:
1) The use of D-aminos in these molecules are due to post-translational modifications of peptides. This is a completely different pathway from protein synthesis. As you prefer to say, "apples and oranges."
2) The mechanism by which antibiotics act supports my position, not yours. The post-translational inclusion of D-aminos acts on the protein synthesis machinery of bacteria. In effect, it jams up the ribosomes prohibiting protein synthesis. So, inclusion of D-aminos appear to have a deleterious effect on bacteria. Again, this hardly supports your position.
3) This particular ability to modify translated proteins into antibiotics developed in eukaryotic fungi, far later in evolutionary time than you propose.
At any rate, I am only interested in evolution and not abiogenesis. My iniitial comment that universal use of L-amino acids is evidence of common descent is supported by observation and experiment. Any claims of the exclusive use of D-aminos is mere speculation and is not based on any observation of reality. You stated this in your previous reply to my first post. I don't understand why you are arguing this point now.
It appears that you are intelligent and possess some common sense. I recommend that you enroll in biology and chemistry courses at your local University so that you can see through the misinformation that Brad McPhall has thus far provided.
One more question: How would one be able to falsify your claim of organisms capable of incorporation of D-amino acids into peptides?

This message is a reply to:
 Message 62 by RAZD, posted 07-25-2006 8:07 AM RAZD has replied

Replies to this message:
 Message 80 by RAZD, posted 07-30-2006 11:07 PM Bradcap1 has not replied

  
Bradcap1
Inactive Member


Message 72 of 81 (336633)
07-30-2006 1:24 PM
Reply to: Message 71 by RAZD
07-29-2006 11:37 AM


Re: abiogenesis versus common descent, materials, etc
(bold and underline mine for emPHAsis)
When it comes to abiogenesis I am content to say "we don't know" and include in that "we don't know" that there could have been multiple starts on life, multiple answers to the riddle of replication, etc, before life {settled} on the solution we see about us today.
My initial response to you was that the evidence does not support your position. You have confirmed that this is the case twice. You then take issue with this statement. This does not make sense.
We obviously were discussing abiogenesis.
My initial post was in response to Evopeach. His post implies that chirality poses a problem for evolutionary theory. My response explained that it does not support his position, it in fact supports evolutionary theory.
Nowhere in the provided quotebox is abiogenesis obvious or implied.
The evidence we have today for genetic etc universal use of L-amino acids is a very small subset of all the evidence of use by all organisms since life on earth began. Thus {L-aminos} are evidence that does not refute {common descent} but it also does not prove {common descent}. Even if you could show that NO forms of life could use {D-aminos} this would not be evidence that would prove {common descent}.
I will state again that the evidence does not support this position. Unless you can provide it, you must concede this point.
It - alone - is not conclusive evidence of {ultimate common descent}, just of common materials. You could have common descent with {D-amino} and {both amino} and you would still have common descent. You could have two or more different lines of {recent common descent} that (all) happen to use {L-amino} at their start, and you would not have evidence for {ultimate common descent}. Even if "recent" was every thing since 488 million years ago (ie since Cambrian\Ordovician Extinction event}.
It appears the you've been ignoring my explanations of how gene expression works. DNA and RNA are both directional molecules. They produce proteins that incorporate L-amino acids into nascent peptides. Thus 5'-3' right handed helical DNA will always result in the incorporation of L-aminos. Without modifying the genetic code in a drastic way, so drastic that vestiges of it would remain today, D-aminos cannot be incorporated into peptides. Organisms that evolved mechanisms to make post-translational modifications of petides by adding d-aminos and/or polysaccharides are also evidence of common descent. I have also demonstrated that there are 41 codon sequences that are redundant for L-aminos. Not one of them code for D-aminos.
Now, the complementary base-pairing properties of DNA are the ultimate evidence of common descent. Before DNA sequencing became routine, genes that were characterized in model organisms like Drosophila and C. elegans were used to find homologous genes in other organisms including man. In an assay called a zoo blot, a fragment of a gene from the model organism is labeled with a radioactive label and hybridized to the DNA of the organisms whose gene you are trying to find. For example, the sequence 5'-GACT-3' would hybridize only to the sequence 3'-CTGA-5'. In reality the probe is going to be a longer sequence. The longer the sequence, the more specific the probe becomes. At each position on the DNA stand there are 4 posible bases (1 in 4). The odds of a 40 base sequence is 1 in 4^40. As you can see, these probes are very specific.
Today, DNA sequencing is routinely done and complete genomes of numerous organisms have been sequenced. The average size of the coding region of a gene is 2500 bases. The possibilty of thes genes arising from an independent event is 1 in 4^2500. Yet we find these genes time and time again. Examples of this are hox genes, globin genes, distalless, pax6, and thousands more. The gene for pax6 has even been removed from Drosophila and replaced with the Pax6 gene from mice and restored eye formation that was lost when the gene was removed.
Pseudogenes that exist across genomes reinforce the concept of common descent as does high conservation of sequence in exons and low conservation in introns.
The tree of life you are talking about is one based on the assumption of {common descent} so it is not evidence for common descent, just of the logical conclusions based on it. Using a conclusion from {common descent} as evidence for {common descent} is either {begging the question} or {circular reasoning} -- logical fallacies again.
You can't be serious. Please go take some courses and learn the fundamentals of science before trying to take on advanced subjects like this. You have curiousity, that is good. Now do something with it.
The scientific method follows (from http://physics.ucr.edu/~wudka/Physics7/Notes_www/node6.html):
1. Observe some aspect of the universe.
2. Invent a tentative description, called a hypothesis, that is consistent with what you have observed.
3. Use the hypothesis to make predictions.
4. Test those predictions by experiments or further observations and modify the hypothesis in the light of your results.
5. Repeat steps 3 and 4 until there are no discrepancies between theory and experiment and/or observation.
Are you stating that this is logical fallacy?
The problem with ideas you propose is step 1. The phenomena you propose hasn't been observed. You also can't test any predictions you might be able to make (step 4).
The bottom line here is that both you and Mr. Mcphall are capable of intelligent thought, but you lack the education and training in this field to put forth speculation like this. Your ideas are easily dismissed by OBSERVED evidence that is published in hundreds of professional journals.
Please, go get educated in this area, your curiousity and energy would make you a good scientist.
ps - thanks for coming back.
I'm not trying to be a smart-aleck here, but I've been in Atlantic City on a well-deserved vacation and had no idea that I had been banned for a day.

This message is a reply to:
 Message 71 by RAZD, posted 07-29-2006 11:37 AM RAZD has not replied

Replies to this message:
 Message 73 by nwr, posted 07-30-2006 2:45 PM Bradcap1 has replied

  
Bradcap1
Inactive Member


Message 74 of 81 (336665)
07-30-2006 4:28 PM
Reply to: Message 73 by nwr
07-30-2006 2:45 PM


Re: abiogenesis versus common descent, materials, etc
Razd proposes that more than one line could have existed prior to the line that exists now. I stated that there is no evidence to support this view. He has supported my statement by twice admitting that there is no evidence to support his claim.
'm quite sure that RAZD knows very well how gene expression works. But it seems not relevant to the point he was making.
He apparently does not. You apparently do not either. The structure of DNA and RNA results in the incorporation of L-amino acids in peptide synthesis. This is apparently escaping the notice of all of you.
Why don't any of you write up a grant proposal on this and submit it to the NIH or the NSF? How about preparing a doctoral thesis on this ? I'd love to see the response you get.
You might try actually looking up the research that has been done in this area.
As for me, I am quite surprised at the level of scientific illiteracy that is present in this thread.

This message is a reply to:
 Message 73 by nwr, posted 07-30-2006 2:45 PM nwr has seen this message but not replied

Replies to this message:
 Message 75 by Belfry, posted 07-30-2006 5:06 PM Bradcap1 has replied

  
Bradcap1
Inactive Member


Message 76 of 81 (336741)
07-30-2006 8:17 PM
Reply to: Message 75 by Belfry
07-30-2006 5:06 PM


Re: abiogenesis versus common descent, materials, etc
Absolutely not. Razd and I agree on this. However, he/she bristled when I suggested in my first response that there was no evidence to support the claim.
The point that is at issue now is that I have pointed out that the preference of living organisms for L-aminos is in fact due to the genomes of said organisms. Razd then stated that the use of DNA as genetic material could be due to common materials and not common descent (at least, this is my understanding of his post). I understood that the position of Razd is that homology between genomes is not evidence of common descent. My examples of assays based on complementary base pairing properties of DNA and homologous genes based on DNA sequencing are meant to support the position that conservation of sequence is evidence of common descent.
I was not trying to be combative to Razd or to misinterpret his/her post. If I did, Razd please accept my apologies.
Anyway, my vacation is over and its time to get back to work.
I tried to convey to Razd that I respect the effort that is being made to understand the subject.

This message is a reply to:
 Message 75 by Belfry, posted 07-30-2006 5:06 PM Belfry has not replied

  
Bradcap1
Inactive Member


Message 77 of 81 (336744)
07-30-2006 8:28 PM
Reply to: Message 75 by Belfry
07-30-2006 5:06 PM


Re: abiogenesis versus common descent, materials, etc
RAZD and NWR don't appear to be disagreeing with you on anything. So, sarcastic suggestions that they submit a "claim" for publication are rather out of the blue, as they are making no controversial claim, as far as I can see.
Controversial claim #1:
There could have been any number of lines previous to the one that exists now.
See the scientific method in my previous post and the problems associated with this claim.
Controversial Claim #2:
Homology between genomes is not evidence of common descent.
See assays based on complimentary base pairing and the discipline of bioinformatics (comparative genomics), as well as any of the thousands of published research papers reporting on homologous genes.

This message is a reply to:
 Message 75 by Belfry, posted 07-30-2006 5:06 PM Belfry has replied

Replies to this message:
 Message 78 by nwr, posted 07-30-2006 8:52 PM Bradcap1 has not replied
 Message 79 by Belfry, posted 07-30-2006 9:00 PM Bradcap1 has not replied

  
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