Animals and humans only. I don't include plants or microorganisms, and I don't think any pain, even of stubbing the toe, existed in Eden.
I'm not trying to play "gotcha", but I did genuinely have a question about this. Pain exists as a signal of injury from the body. Undetected injuries can result in tissue necrosis - this is common in persons who have lost sensation in the extremities, like sufferers of leprosy - and so the body warns us of injury via pain.
How did it work in Eden? If you stub your toe and it doesn't hurt, what prevents you from suffering even greater injury? Or is it just that no injury occurs? Rocks leap out of the way at the approach of your foot?
Like I said I'm not trying to put you on the spot, and I don't intend to press this as a discussion. I was just hoping you'd briefly expand your thoughts on this, if you felt like doing it.